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KMID : 1189420220350010011
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Journal of Korean Oriental Medical Ophthalmology & Otolaryngology & Dermatology
2022 Volume.35 No. 1 p.11 ~ p.23
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Anti-inflammatory Effects of Cistanche deserticola Water Extracts in LPS-stimulated RAW 264.7 Macrophages
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Park Sun-Myoung
Lee Kyou-Young
Hong Chul-Hee
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Abstract
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Objectives : The purpose of this study is to confirm the anti-inflammatory effects of Cistanche deserticola Water Extracts(CDWE).
Methods : In this study, cell viability was determined by MTT assay. NO production was determined with Griess reagent, and IL-6 production was determined by ELISA. And western blot analysis was performed to confirm the protein expression of NOS2, COX-2, IκB-α, p-IκB-α, and NF-κB.
Results : CDWE was not cytotoxic at 15.625-1,000㎍/㎖ in RAW 264.7 cells. CDWE inhibited NO production in a concentration-dependent manner, with inhibitory effect on expression levels of IL-6. Expression level of NOS2, NF-κB p65, and p-IκB-α decreased at 250-1,000㎍/㎖. mRNA expression of COX-2 suppressed at 250, 1,000㎍/㎖.
Conclusions : These results suggest that CDWE has anti-inflammatory effects and can be used as an anti-inflammatory therapeutic material.
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KEYWORD
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Cistanche deserticola, RAW 264.7 cells, LPS, Anti-inflammation, NF-κB
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